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by Rocky S. Tuan

Download Recombinant Gene Expression Protocols (Methods in Molecular Biology) fb2, epub

ISBN: 0896033333
Author: Rocky S. Tuan
Language: English
Publisher: Humana Press; 1997 edition (March 6, 1997)
Pages: 521
Category: Biological Sciences
Subcategory: Science
Rating: 4.2
Votes: 555
Size Fb2: 1768 kb
Size ePub: 1286 kb
Size Djvu: 1406 kb
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Recombinant Gene Expression Protocols.

Recombinant Gene Expression Protocols. The ability to insert specific gene sequences into cloning vectors and their subse­ quent expansion is the cornerstone of modem molecular biology. A direct practical outcome of molecular cloning technology is its application to ex­ press specific recombinant genes.

Such recombinant constructs represent the basic reagents of molecular biology. Cite this protocol as: Tuan . Methods in Molecular Biology, vol 62.

Part of the Methods in Molecular Biology book series (MIMB, volume 62). Abstract. Such recombinant constructs represent the basic reagents of molecular biology. One of the major applications utilizing cloned DNA sequences is the expression of the cloned DNA into a protein product, . the expression of recombinant genes. 1997) Recombinant Gene Expression Protocols. eds) Recombinant Gene Expression Protocols. Humana Press.

Series: Methods in Molecular Biology. Overview of Experimental Strategies for the Expression of Recombinant Genes Rocky S. Tuan 1. Introduction The direct clonmg of DNA fragments, etther dertved from naturally occurrmg or artrficrally designed gene sequences, into various clomng vectors, mcludmg bacteriophages, plasmids, and vu-uses, IS the cornerstone of modern molecular biology.

Includes bibliographical references and index. ISBN 1-58829-262-2 (alk. paper) 1. Genetic tory manuals.

Start by marking Methods in Molecular Biology, Volume 62. .They also provide invaluable detailed information on how to introduce the recombinant gene construct into the appropriate expression host.

Start by marking Methods in Molecular Biology, Volume 62: Recombinant Gene Expression Protocols as Want to Read: Want to Read savin. ant to Read. Read by Rocky S. Tuan.

Recombinant Gene Expression. Book · January 2012 with 4 Reads . How we measure 'reads'. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Recombinant Gene Expression Protocols Tuan Rocky S. Springer .

Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls

The current explosive progress in molecular biological research can be definitively traced to the development of molecular cloning technology. The ability to insert specific gene sequences into cloning vectors and their subse­ quent expansion is the cornerstone of modem molecular biology. A direct practical outcome of molecular cloning technology is its application to ex­ press specific recombinant genes. Currently, recombinant gene products are used in a wide spectrum of applications, including gene therapy, production of bioactive pharmaceuticals, synthesis of novel biopolymers, in agriculture and animal husbandry, and so on. A fundamental requirement for successful recombinant gene expression is the design of the cloning vector and the choice of the host organism for expression. Recombinant Gene Expression Protocols grows out of the need for a laboratory manual that provides the reader the background and rationale, as well as the practical protocols for the preparation of "expression constructs" and their introduction into appropriate host cells and/or organisms. The chap­ ters in this book are grouped by their expression hosts, including E. coli, yeast, mammalian cells, nonmammalian eukaryotes such as plants, Xenopus, and insects, as well as in transgenic organisms. In-depth information is presented on the important characteristics of expression cloning vectors and the various methods for efficiently introducing expression constructs into target cells and/ or organisms. Throughout Recombinant Gene Expression Protocols, the authors have consistently striven for a balanced presentation of both background informa­ tion and actual laboratory details.

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