This paper describes some current work pertaining to transformation of cells by oncogenic viruses. Characterization of transformation by SV40 of cells derived from interspecific hybrid hamsters.
This paper describes some current work pertaining to transformation of cells by oncogenic viruses.
To date, the only viruses that have been shown capable of transforming human cells in vitroare Simian Virus 40. .By currently accepted standards, infection of human tissue with Rous sarcoma or wart viruses has not led to transformation.
To date, the only viruses that have been shown capable of transforming human cells in vitroare Simian Virus 40 (13, 16, 20, 24) and a hybrid strain or adenovirus type 7-SV40 (strain LLE-46) (4. Infectious Virus Simian Virus Contact Inhibition Virus Yield Adenovirus Type.
SV40 has been detected prevalently in a limited panel of human tumors . The apparent different cell tropism could be related to the virus (.
SV40 has been detected prevalently in a limited panel of human tumors: mesothelioma, bone and brain tumors, and lymphoma. These are the same tumor types that are specifically induced by SV40 when injected into hamsters, a finding that has raised concerns about the possible pathogenic role of SV40 in humans. possibly to the number of 72-bp elements) and to different expression of cellular genes, known to play a critical role in SV40-mediated transformation of human cells, such as Notch-1 and c-Met.
Simian virus 40 (SV40) infection of human skin fibroblast and human tumor .
Simian virus 40 (SV40) infection of human skin fibroblast and human tumor cells resulted in the expression of T-antigen and transformed foci. The transformation of these cells by SV40 was also dependent on the multiplicity of infection (MOI), represented by the linear portion of the curve (range about 100-1000 pfu) for both normal and ACR cells. Tissue culture cell strains of foreskin nbroblasts obtained from 5 neonates with Down's Syndrome (trisomy 21-22) were shown to be at least twice as susceptible and, the average, 3 times as susceptible to transformation by the SV40 virus as diploid foreskin nbroblasts from normal neonates.
A quantitative system has been developed for the study of transformation of human diploid fibroblasts in culture by two oncogenic viruses, SV40 and the E46 strain of adeno 7-SV40 "hybrid" virus. Seven of the eleven cell strains derived from human skin biopsies when infected with SV40 (10 9 tissue culture infective doses per milliliter) gave rise to transformed colonies with approximately the same frequency (. 3 percent).
Cell culture is the process by which cells are grown under controlled conditions, generally outside their natural environment. After the cells of interest have been isolated from living tissue, they can subsequently be maintained under carefully controlled conditions.
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SV40 is a highly oncogenic virus in rodent cells and has served as an extremely valuable model for determining the various mechanisms by which DNA tumor viruses transform cells and contribute to tumor formation.
SV40 has been detected prevalently in a limited panel of human tumors: mesothelioma . All viruses transformed astrocytes; only wtSV40 transformed HM. Two different SV40 isolates differing in the number of 72-bp elements in the virus regulatory region, archetypal SV40 (1ESV40), which contains one 72 bp, and nonarchetypal SV40 (wtSV40), which contains two 72 bp, have been detected in human tumors.